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. 2018 Feb 20;7(2):16. doi: 10.1038/s41389-018-0028-8

Fig. 1. Downregulation of miR-214-induced radioresistance of CRC.

Fig. 1

a Differentially expressed microRNAs in SW837 cells in response to IR treatment by microRNA microarray. U6 was used as internal control. b MiR-214 expression in HT29 and LS174.T CRC cell lines by quantitative real-time PCR analysis. Data represent mean ± SD. *p < 0.05, **p < 0.01. c Plasma level of miR-214 in CRC patients after IR exposure by quantitative real-time PCR analysis. **p < 0.01. d Cell viability of Mock and miR-214 expressing SW480 and HCT116 cells detected by CCK-8 assay. Data represent mean ± SD. *p < 0.05, **p < 0.01. e Cell viability in NC and anti-miR-214 expressing Ls174.T and HT29 cells detected by CCK-8 assay. Data represent mean ± SD. *p < 0.05, **p < 0.01. f Clonogenic survival assay of Mock and miR-214-expressing SW480 and HCT116 cells treated by indicated irradiation dosage. Data represent mean ± SD. *p < 0.05, **p < 0.01. g Clonogenic survival assay of NC and miR-214-inhibiting LS174.T and HT29 cells treated by indicated irradiation dosage. Data represent mean ± SD. *p < 0.05, **p < 0.01. h The growth of subcutaneous tumor derived from SW480/Mock and SW480/miR-214 cells in Balb/C nude mice. Mice were treated with 10 Gy irradiation at tenth day post tumor cell injection. N = 5 per group, data represent mean ± SD. *p < 0.05, **p < 0.01