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. 2018 Feb 20;7(2):16. doi: 10.1038/s41389-018-0028-8

Fig. 5. ATG12 attenuates miR-214-mediated autophagy inhibition in CRC cells.

Fig. 5

a Relative expression of ATG12 mRNA in Mock, miR-214 and miR-214/ATG12 SW480, and HCT116 cells by real-time PCR. Data represent mean ± SD. **p < 0.01. b Expression of autophagy-related proteins in Mock, miR-214, and miR-214/ATG12-expressing SW480 and HCT116 cells. c IF observation of LC3 puncta in Mock, miR-214, and miR-214/ATG12-expressing SW480 and HCT116 cell exposed to 4 Gy radiation. Data represent mean ± SD. **p < 0.01. d Autophagy evaluation of Mock, miR-214, and miR-214/ATG12-expressing SW480 and HCT116 cell exposed to 4 Gy radiation by TEM. Data represent mean ± SD. **p < 0.01. e Cell viability of Mock, miR-214 and miR-214/ATG12-expressing SW480 and HCT116 cells after IR exposure by CCK-8. *p < 0.05. f Cell survival fractions of Mock, miR-214 and miR-214/ATG12-expressing SW480 and HCT116 cells after IR exposure by plate colony formation. *p < 0.05. **p < 0.01. g The growth of subcutaneous tumor derived from SW480/Mock, SW480/miR-214, and SW480/miR-214/ATG12 cells in Balb/C nude mice. Mice were treated with 10 Gy irradiation at tenth day post tumor cell injection. N = 5 per group, data represent mean ± SD. *p < 0.05, **p < 0.01. h Expression of ATG12, LC3, p62 and Bax, cleaved caspase3, Bcl-2 in Mock, miR-214 or miR-214/ATG12-expressing SW480 cells-derived tumors by Western blot