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. 2018 Feb 14;9(2):241. doi: 10.1038/s41419-017-0255-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative TEM images of MCF-7 cells treated with NBT (12 μM). N nucleus; M mitochondria; red arrows indicate autophagic vacuoles (scale bars: 2 μm, 500 nm). b Total cellular extract of MCF-7 cells treated with 6, 12, and 24 μM NBT was prepared and subjected to Western blot by using antibodies against Atg5, LC3B, and p62. Data represent mean ± SD of three independent experiments. c Cells were transfected with a tandem reporter construct (tfLC3) for 48 h and were exposed to Rapa (0.25 μM), NBT (12 μM), and/or CQ (40 μM) for 24 h. The cells were then stained with Hoechst 33258 fluorescent dye. The puncta were examined by using a fluorescence microscope. Scale bars: 50 μm

Fig. 5 — a Representative TEM images of MCF-7 cells treated with NBT (12 μM). N nucleus; M mitochondria; red arrows indicate autophagic vacuoles (scale bars: 2 μm, 500 nm). b Total cellular extract of MCF-7 cells treated with 6, 12, and 24 μM NBT was prepared and subjected to Western blot by using antibodies against Atg5, LC3B, and p62. Data represent mean ± SD of three independent experiments. c Cells were transfected with a tandem reporter construct (tfLC3) for 48 h and were exposed to Rapa (0.25 μM), NBT (12 μM), and/or CQ (40 μM) for 24 h. The cells were then stained with Hoechst 33258 fluorescent dye. The puncta were examined by using a fluorescence microscope. Scale bars: 50 μm