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. 2018 Jan 18;9(2):33. doi: 10.1038/s41419-017-0040-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a RAW264.7 cells pretreated with DMSO or the indicated concentrations of DOT1L inhibitors (EPZ5676 and EPZ004777) and stimulated with RANKL for 60 h. OCs were fixed and stained for TRAP. b–d OC number and surface area measurement according to TRAP staining results shown in a. e Bone resorption assay: OCs were treated with DOT1L inhibitor or DMSO for 5 days in osteoassay plates. Representative images of toluidine blue staining are shown. f Percentage of resorption area relative to the total area of osteoassay plates calculated on the basis of staining results shown in e. Experimental data are expressed as mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, two-tailed unpaired t-test, compared with the DMSO treatment. DMSO: dimethyl sulfoxide.