Figure 2.

FOXK2 inhibits glioma cell proliferation. (A) The transfection efficiency of siRNA was validated by Western blot analysis and real-time PCR analysis. (B) The CCK-8 assays revealed that downregulated FOXK2 expression promoted proliferation of LN229 cells. (C) A colony-formation assay confirmed that the proliferation rate was increased by FOXK2 knockdown. (D) The transfection efficiency of the plasmid encoding the FOXK2 protein was validated by Western blot analysis and real-time PCR analysis in LN229 cells. (E) The CCK-8 assays revealed that increased FOXF2 expression inhibited proliferation of LN229 cells. (F) A colony-formation assay confirmed that the proliferation rate of LN229 cells was inhibited by FOXK2 knockdown. (G) The transfection efficiency of the plasmid encoding the FOXK2 protein was validated by Western blot analysis and real-time PCR analysis in U373 cells. (H) The CCK-8 assays revealed that increased FOXF2 expression inhibited the proliferation of U383 cells. (I) A colony-formation assay confirmed that the proliferation rate of U373 cells was inhibited by FOXK2 knockdown. *p<0.05; **p<0.01; and ***p<0.001.

Abbreviations: CCK-8, Cell Counting Kit-8; FOXK2, Forkhead box K2; NC, negative control; PCR, polymerase chain reaction; si, small interfering.