Table 1.
Primers used for quantitative real-time polymerase chain reaction
| Gene | Forward (F) and reverse (R) primer (5′→3′) | Accession no |
|---|---|---|
| BMP2 | (F) GCTGGTCACAGATAAGGCCA | NM_001200.3 |
| (R) CAGCATCGAGATAGCACTGAGTTC | ||
| BSP | (F) CAGAGGCAGAAAACGGCAAC | NM_004967.3 |
| (R) TTGTTGTCTTCGAGGTGCCC | ||
| COL1A1 | (F) CTGCACGAGTCACACCGGA | NM_000088.3 |
| (R) AAGCCGAATTCCTGGTCTGG | ||
| RUNX2 | (F) TGAGATTTGTGGGCCGGAGTGG | NM_001024630.3 |
| (R) TCTGTGCCTTCTGGGTTCCCGA | ||
| OPN | (F) GGAAAGCGAGGAGTTGAATGGTGC | NM_000582.2 |
| (R) GGACTGCTTGTGGCTGTGGGTTTC | ||
| ACTB | (F) CCAAAGTTCACAATGTGGC | NM_001101.3 |
| (R) GATGGCAAGGGACTTCCTGT |
Abbreviations: BMP2, bone morphogenetic protein 2; BSP, bone sialoprotein; COL1A1, collagen type I alpha I chain; RUNX2, runt-related transcription factor 2; OPN, osteopontin; ACTB, actin beta.