Fig. 2. The MZF1 3′-UTR contains a putative let-7 target site and MZF1 expression correlates negatively with several let-7 family members and positively with LIN28A in primary breast cancer samples.

a Alignment of 9 let-7 family members to their putative target site in the MZF1 3′-UTR (red). All let-7 family members are predicted to bind to the target site via a 7mer seed region (green), with the exception of let-7e binding with an 8mer seed region. b Correlation plots for the correlation between let-7 and MZF1 RNA expression in primary breast cancer samples included in TCGA data. Pairs that show positive correlation are depicted with red circles. Pairs that show negative correlation are depicted with blue circles. The Spearman correlation coefficient is depicted using the radius of the circle: the larger the coefficient, the larger the radius. c Correlation plots of MZF1 and LIN28A expression in TCGA data (all stages). Highest 6% expressing LIN28A samples have been omitted from the figure to better show the correlation. The correlation is assigned with *** indicating p < 0.001. d Correlation plots of MZF1 and LIN28B expression in TCGA (all stages). e Association between MZF1 and Lin28A protein expression quantified from the same TMAs as described in Fig. 1a–c. The Spearman correlation is significant with p < 0.0001