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. 2018 Feb 7;9(2):166. doi: 10.1038/s41419-017-0252-8

Fig. 1. PrP90-231 induces A1 neuron death.

Fig. 1

a Flow cytometry analysis of cell death. Dead cells in the culture were quantified after 24 and 48 h of treatment with PBS (vehicle) or PrP90-231 (100 and 500 nM), by measuring the percent of cells that incorporate SYTOX green. * P < 0.05 vs. vehicle. b Cell viability was quantified in control cells after 48 and 72 h (treatment with PBS, 0 on abscissae) or PrP90-231 (1 and 5 μM) measuring the percent of live cells by Trypan blue exclusion test. *P < 0.05 and **P < 0.01 vs. PBS. c Mitochondrial viability of A1 cells was evaluated by MTT-reduction assay after 72 h of treatment with PBS (0 on abscissae) and PrP90-231 at concentrations ranging from 0.1 to 10 μM. *P < 0.05 and **P < 0.01 vs. PBS. d Phase-contrast pictures of A1 cells that have been treated with PrP90-231 5 μM for two (middle panel) and four (lower panel) days in comparison with untreated (upper panel). Images has been acquired with C- plan 10 × /0.22 magnification lens. Bar 100 μm. All the data are reported as mean + /− SEM of three independent experiments each performed in quadruplicate