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. 2018 Feb 15;9(3):282. doi: 10.1038/s41419-018-0278-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Transposition is possible through a dual vector system that comprises the transposon containing the transgene flanked by two inverted terminal repeats, and a transposase that mobilizes the transposon. The CAR is integrated into the genome through a cut-and-paste mechanism SB transposon vectors are characterized by the presence of specular IR/DR sequences, target for the transposase. The SB vector contains the gene of interest (CAR). The SB transposase (SB-100×) binds to the IR/DR sequences and cuts the vector to release the transposable portion of DNA. TA sequences in the host DNA act as acceptors of the transposed element. The PB transposon is a mobile genetic element that transposes the gene of interest (CAR) from the vector to the host DNA. The l’hyperactive PiggyBac (Hy7 PB) transposase recognizes the transposon-specific “inverted terminal repeats” sequences (ITRs) located at the ends of the gene of interest. Transposition occurs between two TTAA acceptor sites located in the host DNA