Fig. 3. Expression of ABCG2 was downregulated by fasudil via inhibition of ROCK2/moesin/β-catenin.

a U251R and rG-1 were stimulated by fasudil (0.4, 2, 10 μM) for 24 h, and protein levels of ROCK2, p-ROCK2, moesin, p-moesin (Thr 558), β-catenin, p-β-catenin (Ser 552) were detected by western blot. β-Catenin expressions of nucleus (N) and cytoplasm (C) were detected. b U251R and rG-1 cells were transfected with ROCK2-siRNA, p-moesin (Thr 558), p-β-catenin (Ser 552) and ABCG2 were detected, and nuclear accumulation of β-catenin was examined. c U251 cell was pretreated with LPA, and then cells were stimulated with fasudil, and the expression of ROCK2, p-ROCK2 (Tyr 722), moesin, p-moesin (Thr 558), β-catenin, p-β-catenin (Ser 552) and ABCG2 was determined by western blot. d U251R and rG-1 cells were transfected with moesin-siRNA, and protein levels of moesin, p-moesin (Thr 558), β-catenin, p-β-catenin (Ser 552) and ABCG2 were detected by western blot. e LPA was used to stimulate U251 with or without siRNA of moesin, and the expression of p-ROCK2 (Tyr 722), moesin and ABCG2 was examined by western blot. GAPDH and Lamin A were used as loading control