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. 2018 Feb 7;9(2):190. doi: 10.1038/s41419-017-0251-9

Fig. 5. Fasudil combined with TMZ inhibited U251R cell growth in vivo.

Fig. 5

a TMZ resistance tumor size of nude mice was measured by vernier caliper for 21 days during the treatment time in all groups. b, c Weight of tumor obtained from tumor-bearing mice is shown. d Weight changes of nude mice in four separate groups were measured for 21 days. e Weight of heart, liver, spleen, lung, kidney and brain in four separate groups were measured in day 21. f, g ROCK2, p-ROCK2 (Tyr 722), moesin, p-moesin (Thr 558), β-catenin, p-β-catenin (Ser 552) and ABCG2 expression (total, membrane and cytoplasm) of tumors was determined by western blot and quantified (normalized to GAPDH). h, i The membrane and cytoplasm expressions of ABCG2 were quantified (normalized to flotillin-1 or GAPDH). GAPDH and flotillin-1 were used as loading control. Statistical differences compared with the control group is given as *P < 0.05, **P < 0.01, compared with the TMZ group is given as #P < 0.05, ##P < 0.01, and compared with the fasudil group is given as &P < 0.05, &&P < 0.01