Fig. 6. Fasudil combined with TMZ inhibited C6R cell growth in vivo.

a The survival curve of glioma-bearing rats after various treatments. b Weight changes of TMZ-resistant glioma-bearing rats were measured for 25 days. c H&E-stained brain sections from TMZ-resistant glioma-bearing rats after 25 days (upper). Enhanced MRI was performed for TMZ-resistant glioma-bearing rats at day 24 (lower). d ROCK2, p-ROCK2 (Tyr 722), p-moesin (Thr 558), p-β-catenin (Ser 552) and ABCG2 expression of tumors was determined by IHC (scale bar, 50 μm). ABCG2 expression is also shown on the right column by IHC assay (scale bar, 10 μm). Control group: n = 5; TMZ group: n = 4; Fasudil group: n = 3; TMZ+Fasudil group: n = 7. Statistical differences compared with the control group is given as *P < 0.05, **P < 0.01, compared with the TMZ group is given as ^#P < 0.05, ^##P < 0.01, and compared with the fasudil group is given as ^&P < 0.05, ^&&P < 0.01