Fig. 6. Rescued TRIM36 increased the anti-androgen sensitivity in vitro and in vivo.

a, b Validation of rescued TRIM36 expression after anti-androgen drugs in LNCAP and C4–2 cell. c, d The rescued TRIM36 alters the anti-androgen drugs efficacy by inhibiting prostate cancer cell growth. e C4–2 cells with or without expression of TRIM36 were labeled with GFP and injected orthotopically into nude mice. Two week after implantation, tumors were formed and visualized by fluorescence image. Representative fluorescence images of each group are shown (e1–e4). Sequential in vivo whole body fluorescence imaging of tumor progression in different groups. After another 3 weeks and a final fluorescence imaging, mice were sacrificed and tumors were removed (e5–e8). f Tumor sizes in the four groups after sacrifice and tumor removal. *P < 0.05, **P < 0.01