Fig. 6. PPIL2 inhibited metastasis in vivo.

a Mice injected with PPIL2-overexpressing 4T1/Luc cells showed a significant reduction of lung metastases compared to injection with 4T1-pc3.1 cells. n = 5 mice per group. b The weight of lungs in the 4T1-PPIL2-injected group was also significantly decreased compared to the 4T1-pc3.1-injected group. Each bar represents the mean ± S.D. from five independent experiments. *P < 0.05. **P < 0.01. c The level of Flag-PPIL2 in lung tissue was examined to ensure that 4T1/Luc cells had migrated to the lung. d Mice injected with CsA (25 mg/kg/day, n = 8) showed a significant reduction of lung metastases compared to injection with equal volume of 10% DMSO (n = 5). Each bar represents the mean ± S.D. from five independent experiments. *P < 0.05. **P < 0.01. e The lung metastases and endogenous PPIL2 expression in the mice injected with CsA in mouse lung tissue. The lungs tissue slices were observed after hematoxylin–eosin staining (4×). f The bar graphs show the quantitative expression of PPIL2 in e