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. 2018 Feb 8;9(2):200. doi: 10.1038/s41419-017-0173-6

Fig. 5. Chromatin conformation in GATA2 locus is significantly changed upon ATRA induction.

Fig. 5

a Diagram showing ChIP-seq, ATAC-seq, and 4C-seq results of GATA2 promoter and upstream regions. (upper panel) HindIII cutting sites, gene annotation, and control-specific ATAC-seq peaks. Transcription factors with enriched motifs in the peak are labeled. (middle panel) Genome browser views of ChIP-seq and ATAC-seq results of control and ATRA-treated HL-60 cells; semitransparent cyan color labels the bait site used in 4C-seq, and semitransparent yellow color labels the putative enhancers recognized in 4C-seq. (lower panel) 4C-seq results of control and ATRA-treated HL-60 cells using the bait mentioned above. Red dots indicate 4C-seq RPMs in the ATRA-treated cells, and blue dots indicate RPMs in the control cells; only fragments with significant interactions with the bait are shown. Log2-foldchange of RPMs is shown in the lowest bar graph. b DNA FISH showing the loss of the chromatin loop between the GATA2 promoter and enhancer. BAC probes containing the promoter (red) and enhancer (green) were more separated in the ATRA-treated cells (right) than in the control cells (left). c Loci of promoter and enhancer interacted more in the control cells than in the ATRA-treated cells. p-Value was determined by Mann–Whitney U-test: **p-value < 0.001, ***p-value < 0.0001