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. 2018 Feb 9;9(2):202. doi: 10.1038/s41419-017-0193-2

Fig. 1. TMZ–POH downregulates MGMT protein in a concentration-dependent and time-dependent manner.

Fig. 1

a MGMT protein level was evaluated by western blots in A549, SPC-A1, H460, and H520 cells. b MGMT protein level was detected in A549, SPC-A1, and H460 cells following treatment with 100 μM TMZ, POH, TMZ + POH (100 μM TMZ + 100 μM POH), TMZ–POH, or DMSO for 48 h, respectively. c H520 cells were infected by Ad-MGMT or control for 24 h followed by 100 μM TMZ–POH treatment for indicated time. MGMT protein level was detected by western blots. d MGMT protein level was detected in A549, SPC-A1 cells following treatment with 0, 12.5, 25, 50, 100 μM TMZ–POH for 48 h, respectively. e MGMT protein level was detected in A549, SPC-A1 cells treated with 100 μM TMZ–POH for 0, 4, 8, 12, 24 h, followed by washing three times with PBS and incubation in drug-free media for next 24, 48, 72 h, respectively