Fig. 4. TMZ–POH downregulates MGMT independent of Wnt/β-catenin signaling.

a A549 and SPC-A1 cells were treated with 100 μM TMZ, POH, TMZ + POH, TMZ–POH, or DMSO, respectively, for 48 h and subjected to western blot analysis to demonstrate active β-Catenin, β-Catenin, Pho-GSK-3β, ACTB protein level. b A549 and SPC-A1 cells were treated with 0, 100 μM TMZ–POH for 24 h with or without presence of 10 mM LiCl, and western blot analysis demonstrated pho-GSK-3β, MGMT, and ACTB expression