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. 2018 Feb 13;9(2):216. doi: 10.1038/s41419-017-0065-9

Fig. 8. Deltarasin induced the accumulation of ROS and NAC reversed deltarasin-induced apoptosis.

Fig. 8

. (A) A549 and H358 cells were treated with vehicle, 5 μM deltarasin, 5 mM NAC, or in combination of both for 24 h, then cells were stained with DCF-DA and the levels of ROS were analyzed by flow cytometry. (B) A549 cells were treated with vehicle, 5 μM deltarasin, 5 mM NAC, or in combination of both for 24 h and the levels of PARP cleavage and LC3 were evaluated by western blot. (C) A549 cells were treated with vehicle, 5 μM deltarasin, 5 mM NAC, or in combination of both for 24 h and the percentage of cell viability was measured by MTT assays. (D) A549 and H358 cells were treated with vehicle, 5 μM deltarasin, 5 mM NAC, or in combination of both for 24 h, and the levels of cell apoptosis were measured by Annexin V/PI double staining with flow cytometry. The results were expressed as the mean ± SD of three independent experiments, **P < 0.01, ***P < 0.001, when compared with control or deltarasin-treated alone group