Fig. 6. Hpx-induced microglia promoted the viability and the maturation of cultured OPCs.

a–e OPCs were incubated with conditioned medium (CM) from MG^LPS, MG^LPS+HPX, MG^Hpx, or unstimulated microglia (control) for 48 h and then analyzed using the TUNEL assay. f Quantification of the percentage of apoptotic cultured OPCs using the TUNEL assay. Scale bar = 40 µm. g–k Effects of the CM from microglia pretreated with the indicated compounds. Effects of Hpx-induced microglia pretreated with or without LPS on OPC differentiation were determined using an anti-MBP antibody (red). Control, not stimulated; triiodothyronine/thyroxine (T3/T4), positive control. l Histogram showing the percentage of MBP+ OPCs. Data are presented as the means ± SEM of three independent experiments. *p < 0.05, ***p < 0.005 compared with the indicated control. Scale bar = 25 µm