Fig. 3. UHRF1 depletion does not impair DNA damage sensing.

a Immunostaining for γH2AX as an indicator for DNA damage. Y79 shCTL and shUHRF1 cells were pre-treated with either vehicle or 10 µM ATM inhibitor (ATMi) for 2 h, followed by the treatment with 10 µM etoposide for 2 h. b Quantification of γH2AX-positive cells shown in a. Over 300 total cells per group were evaluated for γH2AX-positivity (>10 foci/cell), and the data represent the mean ± SD from three independent experiments. **P < 0.01, NS not significant