Fig. 4. c-FLIP levels regulates TRAIL sensitivity in TNBC cells.

(A) MDA-MB468 and T47D were incubated with or without glutamine for 24 h and FLIP levels were determined by western blotting. (B) MDA-MB468 cells were transfected with siRNA of FLIP or Scrambled (SCR) oligonucleotide for 30 h and then treated with or without TRAIL (100 ng/ml) for 24 h. Apoptosis was measured as described in Materials and Methods section. FLIP levels were determined by western blotting. Error bars represent s.d. from three independent experiments. ***P < 0.001. (C) MDA-MB468 cells were incubated in the presence or absence of glutamine for the indicated times and FLIP levels were determined by western blotting. GAPDH was used as a protein loading control (D) Protein synthesis was measured in MDA-MB468 cells after glutamine deprivation for the indicated times, as described in the Materials and Methods section. Error bars represent SD from three independent experiments. ***P < 0.001