Figure 7.
a) AlamarBlue® activity after 7, 14 and 28 days in culture in Saos-2 treated with NaF for the indicated time periods. b) Alkaline phosphatase activity per mg protein after 7, 14 and 28 days in culture for Saos-2 treated with NaF for the indicated time periods. * significantly higher levels in the indicated group at the same time point compared to cultures treated for days 0-7. † significant compared to cultures treated for days 0-14. ‡ compared to cultures treated for days 7-14. # compared to cultures treated for days 0-28. § compared to cultures treated for days 14-28.