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. 2018 Feb 20;14(2):e1007235. doi: 10.1371/journal.pgen.1007235

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© 2018 Rodriguez et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Accumulation of DNA damage in camta 3–1 is dependent on the NLR signalling component EDS1 and on the NLR DSC2. (A) Representative pictures of comets and (B) % tail DNA quantification of the genotypes. Values are of 3 biological replicates made of pools of different individuals (at least 50 comets scored per biological replicate). Bars marked with different letters are statistically different (P≤ 0.01) among samples according to a Holm-Sidak multiple comparison test. (C) Immunoblot of histone extraction from Col-0, camta3 and camta3 expressing DN-DSC2 probed with anti γ-H2AX antibody. Unspecific band was used as loading control. (D) Quantification of the immunoblot of (C) γ-H2AX analysis normalized to input and to Col-0 (set to 100, values are mean ± SD of 2 biological replicates).