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. 2018 Feb 20;14(2):e1007235. doi: 10.1371/journal.pgen.1007235

Fig 4. ETI activation leads to DNA damage accumulation even in the absence of pathogens.

Fig 4

Recognition of a single effector (avrRPM1) is sufficient to induce DNA damage accumulation. (A and B) Col-0 accumulated more DNA damage than rpm1-3 mutants infected with P. fluorescens harboring avrRPM1. (C and D) In planta expression of avrRPM1 under control of a DEX inducible promotor is sufficient to cause DNA damage (8h after treatment). (A and C) Representative pictures of comet assays and (B and D) Tail DNA % quantification of the genotypes and conditions described. Values of 3 biological replicates made of pools of different individuals (at least 50 comets scored per biological replicate). Bars marked with different letters are statistically different (P≤ 0.01) among samples according to a Holm-Sidak multiple comparison test. (E) Immunoblot of samples of plants sprayed with DEX after the given time points probed with anti γ-H2AX antibody. (F) Quantification of the immunoblot of (C) γ-H2AX analysis normalized to input and to 0h sample (set to 100) (Values are mean ± SD of 2 biological replicates).