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. 2018 Feb 20;14(2):e1007235. doi: 10.1371/journal.pgen.1007235

Fig 7. DNA Damage Repair shutdown is dependent on ETI signalling components.

Fig 7

(A to D) RAD51 accumulates in an immunity dependent manner. (A) sni1 displays enhanced accumulation of RAD51 which is reduced in sni1 eds1-2. Immunoblot of total protein extracts from eds1-2, sni1, sni1 eds1-2 probed with anti RAD51 antibody. An unspecific band was used as loading control. (B) Quantification of the immunoblot of (B) RAD51 normalized to input and to Col-0 (set to 100) (Values are mean ± SD of 3 biological replicates). (C) ETI activation causes RAD51 degradation by proteases with Caspase 3-like activity. Protein extracts from plants subjected to the conditions given were probed with anti RAD51 antibody. Unspecific band was used as loading control. (D) Quantification of the immunoblot of (C) RAD51 normalized to input and to Col-0 infected at 2h (set to 100) (Values are mean ± SD of 3 biological replicates). (E and F) DDR genes are downregulated during HR PCD, (E) downregulation of DDR genes in camta 3–1 and sni1 is dependent of EDS1. (F) vad1 and pub 13, but not dnd1, display DDR gene downregulation. Gene expression was normalized to UBQ10 and is relative to Col-0 (values represent the average ± SD of 3 biological replicates).