Skip to main content
. 2018 Feb 20;14(2):e1007237. doi: 10.1371/journal.pgen.1007237

Fig 1. Expression of CSN gene products and CUL1 modification status in various viable csn mutants.

Fig 1

(A) Apical apex tissues from indicated mutants were extracted and the proteins examined by immunoblotting using indicated antibodies. Lower levels of the CSN (CSN3 and CSN5A) and hyper-neddylation of CUL1 were found in csn5a-1, csn5a-2, csn1-10, and csn2-5. (B) Light-grown seedlings (4-day) of indicated genotypes were used for immunoblotting with anti-CSN5B. The antibody strongly reacted with CSN5B, but also cross-reacted with CSN5A. Endogenous CSN5B migrated slower than CSN5A on the SDS-PAGE. See also S1 Fig.