Fig 3. The germination defects of csn1 and csn5a are seed-coat dependent.

Intact seeds or embryos dissected from the same seed population were germinated on water-agar plates. (A) csn5a-1, or (B) csn1-10, with the corresponding wild type Col seeds as the controls. (C) csn1-1 (fus6-1) mutant seeds were picked out from the heterozygous (+/-) seed population based on the dark purple seed color, and normal colored seeds were used as the positive control (WT). While the seeds of the csn mutant could not germinate on time, their embryos showed normal progression of growth, with radicle elongation on day-2 and cotyledon greening and expansion on day-3. csn1-1 mutants arrested the growth after germination.