(A and B) Germination of csn1-10 abi5-4
(A) or csn5a-1 abi5-4
(B) double mutants displayed normal rate, in contrast to slow germination of the corresponding single mutant csn1-10 and csn5a-1, respectively. All of the csn1-10 abi5-4 lines including double mutant db-5, wild type (WT), csn1-10, and abi5-4 are segregating sibling lines from the same genetic cross. Likewise, all of the csn5a-1 abi5-4 lines including the double mutant csn5a-1 abi5-4, wild type (WT), csn5a-1, and abi5-4 are segregating sibling lines from the same genetic cross. Error bars represent standard deviation from 4 repeats. (C and D) Anti-RGL2 immunoblots showing that timely removal of RGL2 was restored in csn1-10 abi5-4 double mutant (C) as well as in csn5a-1 abi5-4 double mutant (D). Samples of indicated lines were taken at indicated times after seed imbibition. See also S5 Fig.