Skip to main content
. 2018 Feb 20;14(2):e1007216. doi: 10.1371/journal.pgen.1007216

Fig 8. INO80 is an effector of the TORC1 pathway.

Fig 8

(A) Violin and box plots of log-transformed expression fold-change after 45 minutes of 30 nM rapamycin (Rap) treatment compared to untreated cells in the indicated strains. The top and bottom 3% of genome wide responses were excluded for plotting. Significance was determined using a Wilcoxon rank sum test with all genes. (B) Western analysis of phospho-Rps6 (pRps6) reduction following 30 nM rapamycin treatment for indicated minutes (min) in wild-type (WT) and ino80Δ strain. Histone H3 (H3) is a loading control. (C) Fitness assay of deletion strains compared to wild-type (WT). Serial dilution (1:5) of strains were grown at 30°C on synthetic complete (SC) media with 0 or 5nM rapamycin.