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. 2018 Feb 9;7:e33220. doi: 10.7554/eLife.33220

Figure 4. Sleep regulatory mechanisms are distinct between larval and adult stages in Drosophila.

(A) Quantification of sleep in hourly bins demonstrates that sleep amount and distribution are unchanged with rearing of embryos/larvae in constant light (LL; n = 27) compared to normal 12:12 light:dark cycles (LD; n = 29). (B) Sleep is unaffected in molecular clock mutants clkJrk and cyc01 (n = 16,18,21). All sleep assays were conducted in constant dark under infrared light condition. (C) Quantification of larval total sleep over 6 hr in mutants known to be short-sleepers as adults (iso31 controls, sleepless [sss], and fumin [fmn]; n = 27,18,24 larvae from left to right). (D) No correlation is found between larval and adult sleep time (slope of linear regression line is not significantly different from zero). Sleep is sexually dimorphic in adults (Female (F) < Male (M); the red points (F) are distributed on the y axis lower than the blue points (M)), but not in larvae (red and blue points are evenly distributed on the x axis) (M; n = 34; F; n = 25).

Figure 4.

Figure 4—figure supplement 1. Temporal distribution of larval sleep is independent of the circadian clock.

Figure 4—figure supplement 1.

(A,B) Quantification of sleep in hourly bins demonstrates that sleep metrics and patterns are unchanged with rearing of embryos/larvae in constant light (LL; n = 27) compared to normal 12:12 light:dark cycles (LD; n = 29). (C,D) Sleep pattern is unaffected in molecular clock mutants clkJrk and cyc01 (n = 16,18,21). All sleep assays were conducted in constant dark under infrared light conditions.
Figure 4—figure supplement 2. Drosophila larval sleep in adult sleep mutants.

Figure 4—figure supplement 2.

(A,B) The adult short-sleeping mutant sleepless (sss) exhibits similar larval sleep metrics to genetic controls (iso31). The adult short-sleeping mutant fumin (fmn), despite having fragmented sleep in adulthood, actually shows significantly more consolidated sleep (fewer, longer sleep bouts) in larval stages compared to controls, though overall sleep amount is unchanged (Figure 4A) (n = 27,18,23 in A and B).