Figure 1. APE1/Ref-1 and survivin are nuclear and cytoplasmic localized in human prostate cancer.

(A) Hematoxylin and Eosin staining representing non-diseased (peripheral zone taken from cystoprostatectomy) and cancerous human prostate specimens (1–3). Scale bar = 10 µM. Immunofluorescent images of stained non-diseased and cancerous sections (1′-3′) for APE1/Ref-1 (red) and survivin (green). Scale bar = 25 µm, n = 12. (B) Cellular fractionation representing basal survivin and APE1/Ref-1 protein localization in cancerous (PC-3, C4-2 and LNCaP) and non-cancerous (E7) prostatic cell lines. MEK 1/2 (cytoplasmic), Lamin B1 (nuclear) and Histone H3 (chromatin bound) were used as controls for each subcellular fraction