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. 2017 Dec 13;9(13):10962–10977. doi: 10.18632/oncotarget.23493

Figure 4. APE1/Ref-1 siRNA knockdown decreases cell proliferation and survivin protein levels.

Figure 4

(A) Separate aliquots of PC-3 and C4-2 cell lines were transfected with two distinct sequences of 50 nM APE1/Ref-1 siRNA (verified >70% knockdown by immunoblotting) and growth was compared to scrambled siRNA-transfected cells. n = 3, *-denoting p < 0.05 within ANOVA (Scr vs siAPE#1), #- denoting p < 0.05 within ANOVA (Scr vs siAPE#2). (B) Representative pictures of fixed and methylene blue stained C4-2/PC-3 scrambled siRNA (Scr), survivin siRNA #1 (siAPE1 #1) and #2 (siAPE1 #2). (C) Immunoblotting was performed using antibodies for APE1/Ref-1, survivin and GAPDH as labeled after 72 hours post-transfection.