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. 2018 Jan 27;9(13):11159–11169. doi: 10.18632/oncotarget.24329

Figure 3. Estimation of effective NIR-PIT area using the fiber diffuser.

Figure 3

(A) Schema of NIR light transmittance measurement using chicken breast. (B) NIR light transmittance at chicken breast as a function of distance from the fiber diffuser. (C) NIR-PIT regimen. Both BLI and IR700 fluorescence imaging were performed according to this regimen. (D) Bioluminescence images before (-2 hours) and at 1 day after (24 hours) NIR-PIT. Although tumor sizes among each group were approximately the same after NIR-PIT, only the NIR-PIT group mice showed a dramatic decrease in BLI at 24 hours after NIR-PIT. (E) IR700 fluorescence images before and immediately after NIR-PIT. White arrows indicate IR700 accumulation in tumors. Control and Only NIR light groups, exhibited no IR700 fluorescence. The fluorescence decreased in the NIR-PIT-treated mice, indicating NIR light was delivered and resulted in photobleaching of IR700. (F, G) Schemas of NIR-PIT effective area analysis from both BLI and IR700 fluorescence image. The light intensities at each pixel were analyzed as indicated by the arrows, and then compared to before NIR-PIT. (H) Relative luciferase activity compared to before NIR-PIT at each distance from the light source. NIR-PIT group exhibited dramatically decreasing luciferase activity, and significantly differences were seen between 0 – 8.5 mm distance from the light source (n = at least 7; *, P < 0.05 of NIR-PIT group vs. the other groups). (I) Relative IR700 fluorescence ratio comparing before and after NIR-PIT. NIR-PIT group showed significantly decreased IR700 fluorescence intensity within a 10 mm area from fiber diffuser (n = at least 7; *, P < 0.05 vs. Only APC).