Figure 10. Effect of NSAIDs on P-gp-mediated efflux activity of Hsp90 inhibitors in MDR cells.

Cell suspensions were isolated from CEM/VBL₁₀₀ (A and B), MCF7-MDR (C and D) or HeyA8-MDR cells (E and F) treated with 5 μM 17-AAG in the presence or absence of CCB (25 μM for 36 h) or IBU (400 μM for 24 h). And then these are incubated with rhodamine 123 (Rho123) and further incubated at 37°C for 4 h to allow P-gp transporter-mediated efflux. Cellular fluorescences were analyzed immediately by flow cytometry.