Figure 3. Reduction of mutp53 protein levels in MDR cells by NSAIDs.

MCF-7 (A) and MCF7-MDR cells (B) were treated with increasing doses of CCB for 24 h or with 25 μM CCB for the indicated times, and the levels of wild-type (wtp53) and mutant p53 (mutp53) proteins were determined by western blot analysis and. Changes in mutp53 level in CEM/VLB₁₀₀ or HeyA8-MDR cells treated with increasing doses of CCB for 24 h (C) and in three MDR cell lines treated with increasing doses of IBU for 24 h (D) were determined by western blot analysis. MCF7-MDR (E) or CEM/VLB₁₀₀ cells (F) were treated with cycloheximide (CHX, 20 μg/ml for 3∼6 h) in the absence or presence of 25μM CCB. Changes in mutp53 levels were determined by western blot analysis. β-Actin (actin) was used as a loading control.