Figure 8. Enhancement of Hsp90 inhibitor-induced apoptosis and acceleration of autophagic mutp53 degradation and suppression of HSF1/Hsp70 activation of MDR cells treated with Hsp90 inhibitor by NSAIDs.

(A) CEM/VBL₁₀₀ cells were treated with 17-AAG (3 or 25 μM) and/or CCB (10 or 30 μM) for 24 h, and the percentages of viable, early apoptosis and late apoptosis/necrosis cells were assessed by flow cytometry with AnV/IP staining. MCF7-MDR (B and D) and HeyA8-MDR (C and E) were treated with 17-AAG (1 or 10 μM) or AUY922 (10 or 50 nM) in the presence or absence of 25 μM CCB (or 300 μM IBU) for 24 h. Change levels of mut p53, HSF1/Hsp70, p62 and cleaved PARP were determined by western blot analysis.