Figure 9. Attenuation of NSAIDs-induced autophagic degradation of mutp53 and apoptosis in MDR cells treated with Hsp90 inhibitor by autophagy inhibition.

CEM/VBL₁₀₀ cells were co-treated with 17-AAG (1 or 10 μM) 25 μM CCB or AUY922 (10 or 50 nM) in the presence or absence of (or 300 μM IBU) for 24 h, respectively, and changed levels of mut p53, HSF1/Hsp70, p62, Bcl-2, Cl cas-3 and Cl PARP were determined by western blot analysis (A and B). CEM/VLB₁₀₀ cells were treated with 10 μM 17-AAG and/or 10 μM CCB (C) or 50 nM AUY922 and/or 300 μM IBU for 24 h (D), and the percentages of early and late apoptotic cells were assessed by flow cytometry with Annexin-V/PI staining. Images shown are representative of three independent experiments. CEM/VLB₁₀₀ cells were co-treated with 25 μM CCB and 10 μM 17-AAG in the presence or absence of 10 μM LY294002 (or 5 μM CQ) for 24 h and changed levels of mutp53, p62 and Cl PARP were determined by western blot analysis (E and F).