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. 2018 Jan 29;9(13):10834–10846. doi: 10.18632/oncotarget.24330

Figure 1. Colon of D1 receptor-deficient mice (D1-ko) and D2-deficient mice (D2-ko).

Figure 1

Real-time quantitative RT-PCR and western blot (WB) analysis of changes in the expression of AT1 receptors, AT2 receptors and AT1/AT2 ratio, and HPLC analysis of levels of dopamine (DA), noradrenalin (NA) and serotonin (5-HT) in the ascending colon of D1-ko A.-C. and D2-ko D.-F. relative to wild-type (wt) controls. Protein expression was measured relative to the β-actin band value and the expression of each gene was measured relative to that of the housekeeping transcripts (β-Actin). PCR and WB results were normalized to the values for wild-type controls (100%). Data are means ± SEM. *p < 0.05 relative to wt controls (Student’s t test).