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. 2018 Jan 24;9(13):11060–11070. doi: 10.18632/oncotarget.24308

Figure 2. CHIR activates Wnt/β-catenin pathway in bladder cancer cell line-derived organoids.

Figure 2

(A) Bar graphs show the relative gene expression of AXIN2 by qRT-PCR in RT4- and 5637-derived organoids treated for 72 hours with DMSO or CHIR of 5 and 2.5 µM for RT4 and 5637, respectively. GAPDH was used as a reference gene, and relative expression was calculated by the 2 ΔΔCT method. **P ≤ 0.01. (B) Western blot analysis shows protein levels of β-catenin, FOXA1, and GAPDH in RT4- and 5637-derived organoids. Organoids were treated for 24 hours with DMSO or CHIR of 5 and 2.5 µM for RT4 and 5637, respectively. Cytoplasmic and nuclear proteins were separated and analyzed. GAPDH and FOXA1 were used as a loading control for cytoplasmic and nuclear protein, respectively.