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. 2018 Mar 2;8:3911. doi: 10.1038/s41598-018-22143-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mahanine-treated MIAPaCa2 cell exhibited increased intracellular Ca²⁺. (A) Histogram plot of the mean fluorescence intensity of intracellular Ca²⁺ pool as measured by Fluo-3/AM in MIAPaCa-2 cells treated with mahanine (20 µM) at different time points. (B) A representative Fluo-3/AM MFI (mean fluoresce intensity) spectra in mahanine-treated MIAPaCa-2 cells. EGTA (10 mM) and Ca²⁺ ionophore (2 µM) were served as the negative and positive controls respectively. (C) Measurement of Ca²⁺ leakage mediated by ROS in MIAPaCa-2 and BxPC-3 cells pretreated with NAC (ROS inhibitor, 1 hr) followed by mahanine and Ca²⁺ ionophore with indicated dose for 30 min by FACS.