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. 2018 Mar 2;8:3911. doi: 10.1038/s41598-018-22143-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced glycosylation in mahanine treated-MIAPaCa-2 cells. (A) Cell surface expression of glycans was determined using different lectins by FACS as described in material and methods. Histogram plot of mean fluorescence intensity (MFI) of different lectins binding towards the MIAPaCa-2 cells treated with different doses of mahanine for 18 hr. (B) Evaluation of cellular sialoglycoprotein using two sialic acid binding lectins (SNA and MAA) in the MIAPaCa-2 cells treated with mahanine (Mhn, 20 µM) for 18 hr as described in material and methods. In parallel thapsigargin (Tg, 1.0 µM), staurosporine (St, 1.0 µM) were always used for comparison.