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. 2018 Mar 2;8:3933. doi: 10.1038/s41598-018-22194-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Protein expression in abdominal subcutaneous adipose tissue. Expression of proteins involved in the lipolytic pathway (ATGL, n = 12, (A); HSL, n = 13, (B); HSL serine 660 phosphorylation, n = 13, (C), the natriuretic peptide signaling pathway (NPR-A, n = 12, D) and mitochondrial oxidative metabolism (total OXPHOS, n = 13, E) expressed as fold change relative to baseline for each treatment arm (Sacubitril/valsartan: LCZ696 or Amlodipine: AMLO). Data are expressed as mean ± S.E.M. White bars: baseline values; black bars: post-treatment values. Representative (cropped) western blots of which membranes were probed with antibodies directed against total ATGL, total HSL, phosphorylated HSL (p-HSL) on Ser660 and NPRA (F) and uncropped western blots of OXPHOS protein expressions (G).