Fig. 3. 2-APB inhibits BZM-induced autophagy in H1975 and A549 NSCLC cells.

Cells were co-treated with BZM and 2-APB for 24 h following 2-APB pre-treatment for 2 h as indicated. a, b 2-APB decreased LC3-II puncta formation induced by BZM in H1975 and A549 cell lines, respectively. Representative micrographs of cells with endogenous LC3-II puncta and the percentages of cells with increased LC3-II puncta formation are displayed in the upper and bottom panels, respectively. Scale bar = 25 µm, ×60. c, d 2-APB reduced LC3-II conversion induced by BZM in H1975 and A549 cell lines respectively. Representative immunoblots and the LC3 conversion quantification are shown in the upper and bottom panels, respectively. Data are mean value ± S.D. (n = 3). *P < 0.05, **P ≤ 0.01, ***P ≤ 0.001, one-way ANOVA analysis