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. 2018 Mar 2;9(3):361. doi: 10.1038/s41419-018-0397-0

Fig. 4. 2-APB inhibits basal autophagy in HeLa, H1975, and A549 cancer cells.

Fig. 4

After pre-treatment of 2-APB for 2 h, the cells were then co-treated with HCQ and 2-APB for another 24 h. a 2-APB reduced LC3-II puncta accumulated by HCQ in HeLa cell line. Representative micrographs of cells with endogenous LC3-II puncta and the percentage of cells with increased endogenous LC3-II puncta formation are shown. DAPI was used for counterstaining the nucleus. Scale bar = 25 µm, ×60. b 2-APB decreased LC3-II conversion accumulated by HCQ in HeLa cells. Representative immunoblots and the LC3 conversion quantification are displayed. c, d 2-APB reduced LC3-II conversion accumulated by HCQ in H1975 and A549 cell lines, respectively. Representative immunoblot and LC3 conversion quantification are shown; n = 3; **P ≤ 0.01, ***P ≤ 0.001, one-way ANOVA analysis