Fig. 5. 2-APB inhibits BZM-mediated autophagy through intracellular Ca²⁺mobilization suppression in NSCLC cells.

a, b Intracellular Ca²⁺ dynamic changes were performed in H1975 and A549 cell lines, respectively. Cells were loaded with FLIPR Calcium 6 dye and treated as indicated. Real-time Ca²⁺ kinetic was monitored with FLIPR Tetra instrument. Data represent mean values of four independent samples. c, d BAPTA/AM reduced LC3-II conversion induced by BZM in H1975 and A549 cell lines respectively. Cells were co-treated with BZM and BAPTA/AM for 24 h following BAPTA/AM pre-treatment for 2 h as indicated. Representative immunoblot and LC3 conversion quantification are shown; n = 3; **P ≤ 0.01, one-way ANOVA analysis