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. 2018 Mar 2;9(3):361. doi: 10.1038/s41419-018-0397-0

Fig. 6. BZM and 2-APB concomitant treatment suppresses tumor growth in LLC-1 bearing C57BL/6 mouse model.

Fig. 6

After tumor establishment, mice were treated with BZM [0.3 mg/kg/3 days] and/or 2-APB [1 or 2 mg/kg/day] combinations via I.P. injections as indicated for 20 days (n = 10, both male and female). a–c Simultaneous treatment of 2-APB [2 mg/kg/day] and BZM enhanced tumor growth inhibition. Mice tumor volume monitorization, statistics of tumor volume after drug administration, and mice tumor weight are displayed respectively. d 2-APB increased BZM treatment efficacy through autophagy inhibition. Tumor tissues lysates were analyzed by western blot for LC3, Beclin-1, and β-actin (as loading control). e BZM in combination with 2-APB improved the survival rate of C57BL/6-LLC-1 bearing mice compared with BZM group. The lifespan data was collected over the course of the experiment. f Mice body weight monitorization is shown. All data represent mean ± SEM. *P < 0.05, **P ≤ 0.01, ***P ≤ 0.001, Student’s t-test