Fig. 2. Astrocytic JWA deletion decreases the number of TH-positive neurons and exacerbates MPTP/p-neurotoxicity in mice.

a Immunohistochemical staining and quantitative data for TH-positive neurons in the SNc (n = 4). Scale bar = 200 µm. b-d Levels of DA, DOPAC, and HVA were analyzed by HPLC coupled to electrochemical detection in striatum tissues (n = 8). e Western blotting and quantification for analysis of TH protein expression in midbrain extracts. GAPDH was used as a loading control to confirm that equal amounts of protein were loaded in each line (n = 7). Data are presented as mean ± SEM, one-way ANOVA, *P < 0.05, **P < 0.01