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. 2018 Mar 2;9(3):352. doi: 10.1038/s41419-018-0381-8

Fig. 7. JWA regulates the expression of GFAP by transcription factor STAT3.

Fig. 7

a Immunofluorescence for GFAP (red) was immunostained with brain regions STR, SN, HY, PAG, and HP. Scale bars = 200 μm. b C8D1A cells were transfected with different levels of si-JWA (20, 40, 60, and 100 pmol) or Flag-JWA (1, 2, 3 µg) for 48 h, followed by western blotting analysis to detect p-STAT3(Y705), p-STAT3(S727), and STAT3 proteins. c C8D1A cells were incubated with 50 µM of U0126 and/or 20 µM of LY for 3 h after transfect with Flag-con or Flag-JWA for 48 h, followed by western blotting analysis to detect STAT3 (n = 3). GAPDH was used as a loading control to confirm that equal amounts of protein were loaded in each line