Fig. 3.

NBCe1 ion coordination site. a The ion coordination site modeled from the cryoEM densities. Residues important for ion coordination are shown. The circle represents the position of Gly486 in the NBCe1 model. b Na⁺-driven base transport functional mutational analysis of the amino-acid residues located in the ion coordination region. The mean ± s.e.m. is depicted. The number of experiments is shown in parentheses. The residues in this region that are important for ion coordination shown in a and those not important for ion coordination (e.g., Leu479, Thr480, Leu489, Phe491, Val798 etc.) are shown for comparative purposes