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. 2018 Mar 2;9:900. doi: 10.1038/s41467-018-03271-3

Fig. 5.

Fig. 5

Electrophysiological characterization of the NBCe1 483SST-GFS/754D-E/798V-S/800A-T/803I-R mutant (A+B+C). The mutant 483SST-GFS/754D-E/798V-S/800A-T/803I-R construct (NBCe1 Mutant A+B+C), which lost Na+-driven base transport and whose magnitude of Cl-driven base transport approximated AE1, was studied electrophysiologically using whole-cell patch clamping. Unlike electrogenic wt-NBCe1 (b, e, h), the measured I–V curves of the mutant construct (c, f, i) under the various study conditions did not differ from those obtained in mock-transfected cells (a, d, g) demonstrating that the mode of transport of the mutant construct was electroneutral